| 학회 |
한국공업화학회 |
| 학술대회 |
2017년 봄 (05/10 ~ 05/12, 광주 김대중컨벤센센터(Kimdaejung Convention Center)) |
| 권호 |
21권 1호 |
| 발표분야 |
우수논문(구두)_박사과정 |
| 제목 |
Redistribution of flux to glyoxylate for efficient production of 5-aminolevulinic acid in Escherichia coli |
| 초록 |
Microbial production of 5-aminolevulinic acid (ALA) has received much attention. Overexpression along with the deciphering of regulation of the related enzymes and an analogue transporter yielded remarkable achievements in ALA production. Nonetheless, there is significant room for carbon flux optimization to enhance ALA production. The aim of this study was precise carbon flux optimization for high ALA production in Escherichia coli expressing the ALA biosynthetic pathway. Along with the amplification of ALA synthetic pathway, the tricarboxylic acid (TCA) cycle was blocked to force carbon flux toward the ALA production pathway by deletion of sucA. Subsequently, further precise tuning of carbon flux to the glyoxylate cycle by varying the transcriptional strength of aceA led to substantially improved cell growth and ALA production. Thus, this precise tuning of the glyoxylate cycle in a quantitative manner should also enable efficient production of other value-added products. |
| 저자 |
노명현1, 임현규1, 박성훈2, 서상우3, 정규열1
|
| 소속 |
1POSTECH, 2UNIST, 3서울대 |
| 키워드 |
5-aminolevluinic acid; glyoxylate cycle; ALA; metabolic engineering; synthetic biology
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| E-Mail |
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