| 초록 |
Bioreducible polymeric gene delivery carriers can display decreased cytotoxicity and controlled release of genetic materials due to the biodegradation in reductive intracellular environment. Several modifications were performed to the bioreducible polymers. First, arginine or guanidine was introduced as a cell penetrating functionality. Arginine-grafted or guanidinylated bioreducible polymer formed polyplexes with pDNA, releasing pDNA under reductive condition. They showed low cytotoxicity and superior transfection efficiency. It was suggested that GBP possesses strong nuclear localization ability for pDNA delivery. Secondly, 1-(3-aminopropyl) imidazole (API) was introduced as an endosome buffering functionality. Increased endosome buffering capacities proportional to API was evaluated. Increase of API and resultant decrease of arginine residues also reduced cytotoxicities, but decreased pDNA condensing ability, cellular uptakes of polyplex, and finally transfection efficiency as well. |
