| 초록 |
Nucleotides are well-known bioenergy to maintain various cellular functions. Some nucleotide has been used as therapeutics for treating ischemia and liver injury. In this study, we first report endosomolytic functions of nucleotides which exhibit strong proton buffering capacity and/or hemolytic activity in endolysosomal pHs. Nucleotide-containing polymeric gene carriers showed up to 220-fold higher transfection efficiency than nucleotide-free counterpart with no noticeable cytotoxicity. In addition, nucleotides were polymerized to form polynucleotides with or without reducible characteristics. The polymeric derivatives of nucleotides represented strong proton buffering capacities in endolysosomal pH ranges, made complexes with positively-charged proteins, and produced bioenergy. In conclusion, nucleotides and their polymeric derivatives have a new function of endosomolysis for effective drug delivery and would have potentials as biofuels and macromolecular therapeutics and carriers. |
