| 초록 |
Ischemic therapy is based on the stable delivery of angiogenic factors for long-term neovascularization. Here, we developed a new ischemic therapy using engineered mesenchymal stem cells (eMSCs) to express human hepatocyte growth factor (hHGF). The eMSCs were encapsulated for long-term therapeutic effect within self-assembling supramolecular hyaluronic acid (HA) hydrogels. The supramolecular HA hydrogels were prepared by simple mixing in the presence of eMSCs for host-guest interaction between mono-functionalized cucurbit[6]uril - HA (mCB[6]-HA) and diaminohexane conjugated HA (DAH-HA). The angiogenic efficacy was assessed in hind-limb ischemia model mice by laser Doppler imaging, photoacoustic microscopy (PAM), in vivo angiogenic assay, and histological analysis. Especially, the PAM enabled non-invasive imaging of vascular repair process in deep tissue without any contrast agents. Taken together, we could confirm the feasibility of eMSC therapy from the effective vascular repair. |
