| 학회 |
한국고분자학회 |
| 학술대회 |
2020년 가을 (10/05 ~ 10/08, 부산컨벤션센터(BEXCO)) |
| 권호 |
45권 1호 |
| 발표분야 |
신진연구자 특별 심포지움 II |
| 제목 |
Spatiotemporal proximity crosslinking by light activation (Spotlight) for in vivo interactome mapping |
| 초록 |
Proximity labeling is a recently developed method that enables proteomic profiling of specific regions in living cells. However, the diffusive character of in situ-generated reactive species by proximity labeling enzymes cannot be used easily to identify physically interacting partners with the protein of interest (POI). In the present study, we developed an in vivo proteome mapping method that enables spatiotemporal proximity crosslinking by a genetically targeted light-activated ligand of the HaloTag protein in a live mammalian cell (Spotlight). Spotlight was used to reveal the unknown interaction partner of a newly identified mitochondrial protein, exonuclease 3′-5′ domain containing 2 (EXD2) in mammalian cells. |
| 저자 |
강명균 |
| 소속 |
서울대 |
| 키워드 |
Proximity labeling; Spotlight; EXD2; AKAP1
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| E-Mail |
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