| 학회 |
한국공업화학회 |
| 학술대회 |
2016년 봄 (05/02 ~ 05/04, 여수 엑스포 컨벤션) |
| 권호 |
20권 1호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Multiplex microfluidic system integrating sequential operations from microalgal culture to lipid extraction for rapid evaluation |
| 초록 |
Here, we developed a PDMS-based multiplex microfluidic system to expedite multiple steps for lipid sample preparation from different microalgal strains. We could perform sequential operations from cell culture to lipid extraction of different microalgal strains simultaneously without harvesting and purification steps, which are labor- and energy-intensive, by simple injection of medium and solvent into the central inlet. The lipid extraction efficiency using this system was comparable (94.5–102.6%) to the conventional Bligh−Dyer method. We observed that each strain has different lipid accumulation pattern according to stress conditions. These results demonstrate that our multiplex microfluidic approach can provide an efficient analytical tool for rapid analysis of the strain performances (e.g. cell growth and lipid productivities) and determination of optimal lipid induction condition for each strain. |
| 저자 |
곽호석, 김영환, 심상준
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| 소속 |
고려대 |
| 키워드 |
Multiplex microfluidic system; Microalgae; Sample preparation; Lipid induction
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| E-Mail |
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