| 초록 |
Salmonella is the leading pathogenic bacterium which causes the foodborne infection in human and/or animals. Salmonella infection is occasionally fatal, resulting the morbidity and mortality. For Salmonella infection control, disease assessment, and epidemiological surveillance, it is important to determine the exact serotype among more than 2,500 types. In this study, we developed an oligonucleotide microarray-based system for molecular determination of the commonly isolated Salmonella serotypes in Korea. This microarray system employed a single gene as a novel marker, thiamine synthase H subunit (thiH) gene which was found by comparative genome analysis and have the definite ability of the serotype-level identification. Each target serotype of Salmonella can be identified and determined efficiently by the distinctive probe-signal patterns, which can discriminate even the highly closed (related) serotypes. ThiH-based oligonucleotide microarray system can be a useful, robust, and efficient molecular serotype determination tool for Salmonella compared to routine laboratory testing. |
