| 학회 | 한국공업화학회 |
| 학술대회 | 2022년 봄 (05/11 ~ 05/13, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 | 26권 1호 |
| 발표분야 | 포스터-생물공학 |
| 제목 | Rational design of phosphomannoseisomerase from Corynebacterium glutamicumto improve catalytic activity |
| 초록 | Phosphomannose isomerase(EC 5.3.1.8) (PMI)is an enzyme involved in the biosynthesis of high value-added compounds such as GDP-mannose and fucosyllactose. We studied the improvement of the activity of PMI to synthesize mannose 6-phosphate. For this purpose, we applied rational design to construct a mutant enzyme. We predicted binding pose of fructose 6-phosphate at the active site of PMI from Corynebacterium glutamicum. After literature search, we discarded catalytic residues and key residues for substrate recognition. Finally, we selected four active site residues to introduce mutations. We did saturation mutagenesis for these four active site residues. Among the mutant enzyme, L84V mutant showed 25% increased activity. This mutant showed the same thermostability as wild-type enzyme. The reason for the increased activity was elucidated by using protein-ligand docking. |
| 저자 | 이승배, 서주현 |
| 소속 | 국민대 |
| 키워드 | phosphomannose isomerase; fructose 6-phosphate; mannose 6-phosphate |
