| 초록 |
In this study, we propose mechanically enhanced nanovesicles as an effective drug delivery system with increased encapsulated drug stability during the blood circulation in inflammatory disease. We controlled the rigidity of nanovesicles consisted of 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) and 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) by incorporating poly(ethylene oxide)-block-poly(ε-caprolactone)-block-poly(ethylene oxide) (PEO-b-PCL-b-PEO). The PEO chains increase the longevity of nanovesicle during blood circulation as a steric stabilizer, and the PCL chains with high crystallinity provide the membrane rigidity. To evaluate stiffness of nanovesicles, we analyzed deformation using quartz crystal microbalance with dissipation (QCM-D). Finally, we demonstrated enhanced blood circulation time of highly stiff nanovesicles by injection of nanovesicles into rheumatoid arthritis mice model, suggesting its clinical potential as a therapeutic agent. |
