| 학회 |
한국공업화학회 |
| 학술대회 |
2017년 봄 (05/10 ~ 05/12, 광주 김대중컨벤센센터(Kimdaejung Convention Center)) |
| 권호 |
21권 1호 |
| 발표분야 |
정밀화학 |
| 제목 |
Ratiometric Fluorescent Probe Based on a Peptide Receptor with Femtomolar Affinity for Cu(I) in Aqueous Buffered Solutions |
| 초록 |
Fluorescent probes based on novel peptide-based receptors for the detection of Cu(I) were synthesized and characterized. Among them, pyrene-labeled tripeptide probe (Py-WPH) formed a 2:1 complex with Cu(I) and had a femtomolar binding affinity (0.3 fM) for Cu(I) from a competition experiment with bathocuproine disulfonate. Py-WPH detected Cu(I) selectively by a ratiometric response among various biologically relevant metal ions, including Cu(II) and Zn(II). The binding mode study showed that the imidazole group of the peptide receptor plays a critical role in potent binding with Cu(I) and the pi-electrons of indole group of the probe might cooperate to form a complex with Cu(I) The fluorescent peptide-based probe penetrated the living RKO cells and successfully detected intracellular Cu(I) by a ratiometric response. This fluorescent probe based on peptide-based receptor with potent binding affinity for Cu(I) will be a potential tool for better understanding of copper biochemistry. |
| 저자 |
MEHTA PRAMOD KUMAR, 이건형
|
| 소속 |
인하대 |
| 키워드 |
Sensor; Probe; Ratiometric
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| E-Mail |
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