| 초록 |
In this study, we attempt to overcome the problems in the production of CMP-NeuAc by using a recombinant E. coli engineered to co-express CMP-NeuAc biosynthetic enzymes as a biocatalyst. We constructed a recombinant E. coli expressing all the enzymes required for CMP-NeuAc and characterized the process for CMP-NeuAc using the recombinant E. coli as an enzyme source. In addition, we tried to produce sialic acid from N-acetylglucosamine (GlcNAc) using recombinant E. coli co-expressing GlcNAc-2-epimerase and sialic acid aldolase. |
