| 학회 | 한국고분자학회 |
| 학술대회 | 2005년 가을 (10/13 ~ 10/14, 제주 ICC) |
| 권호 | 30권 2호 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Preparation and Characterization of Gradient Polyethylene Surface with Quantum-dots |
| 초록 | A large number of research groups have recently studied the interaction of biomedical materials with biomolecular because are very important as an analytical tool for many biomedical applications. The surface modification of materials was expected to be particularly useful in the design of biomimetic materials modified by bioactive molecules with biomolecular recognizable properties. A method for preparing wettability gradients on various polymer surfaces has been developed by Lee et al. and Pitt et al. The wettability gradients were produced through radio-frequency plasma and corona discharge treatment according to exposing the polymer sheets. [1] Quantum-dots (QDs) was made from a nanometer-scale crystal of semiconductor materials with the optical properties of the materials. The surface chemistry dictated many of the important properties of the QDs nanocrystal in a biological experiment. QDs nanocrystals have been coupled to streptavidin directly through an active ester coupling reaction. This yields a material with streptavidin covalently attached on the surface, which results in QDs Streptavidin Conjugates with high specific biological activity. The gradient polyethylene (PE) surface was prepared by a corona treatment with power increase along the PE length, and successively, biotinylated gradient PE surface was prepared by the reaction of Polyethylenimine (PEI) and biotin. Then QDs treated using PBS buffer solution on PE surface. Surface properties of the gradient PE surface changed by means of PE length and chemical modification. QDs gradient PE surface provided a continuous gradient of QDs' streptavidin through binding of biotin. True-color fluorescence images of CdSe/ZnS QDs emitting single-color were obtained with an Olympus digital color camera in the near-UV range (330-385nm). The luminosity of fluorescence that corresponds to QDs on the biotinylated gradient PE surface increased gradually on the surface along the PE length. Acknowledgement : This work was supported by KMOCIE (N11-A08-1402-05-1-3). Reference 1. J.H. Lee, G. Khang, J.W. Lee, H.B. Lee, Interaction of Different Types of Cells on Polymer Surfaces with Wettability Gradient, J. Colloid Interface Sci. 205, 323-330 (1998) |
| 저자 | 조영호1, 김문석2, 강길선1, 이해방2 |
| 소속 | 1전북대, 2한국화학(연) |
| 키워드 | gradient; Quantum-dots; biotin; streptavidin |
