화학공학소재연구정보센터
학회 한국고분자학회
학술대회 2010년 봄 (04/08 ~ 04/09, 대전컨벤션센터)
권호 35권 1호
발표분야 의료용 고분자 부문위원회
제목 Effect of Purified Alginate Microcapslues on the Articular Cartilage Tissue Engineering : In Vitro Test
초록 A variety of tissue engineering approaches are motivated by the clinical need for articular repair. Alginate has been used as a biomaterial for cartilage regeneration. Purified alginate microcapsules is fabricated by seed cartilage cell into microcapsules. After 1, 3 , 7 day of cell seeding, cell proliferation activity was measured via MTT assay. Morphological observation, histology, biological assay for collagen type Ⅰ, Ⅱ and aggrecan, and PCR were performed at each time point 1, 2 , 3 weeks. In result, when we cultured cartilage cell of rabbit in vitro, we observed better to keep the characteristic of cartilage cell in the Purified alginate microcapsules than Non-purified alginate microcapsules. This study suggests that Purified alginate microcapsules may serve as a potential cell delivery vehicle and a structural basis for in vitro tissue engineered articular cartilage. This research was supported by WCU (R31-20029).
저자 황지혜, 김온유, 배지연, 이동원, 강길선
소속 전북대
키워드 Alginate; Purification; Cartilage; in vitro
E-Mail gskhang@jbnu.ac.kr