| 학회 |
한국화학공학회 |
| 학술대회 |
2007년 봄 (04/19 ~ 04/20, 울산 롯데호텔) |
| 권호 |
13권 1호, p.422 |
| 발표분야 |
생물화공 |
| 제목 |
Microcontact Printing of Biotin for Selective Immobilization of Streptavidin-Fused Proteins and Cells |
| 초록 |
A simple procedure is described for patterning biotin on a glass substrate and then selectively immobilizing proteins of interest onto the biotin-patterned surface. Microcontact printing (μCP) was used to generate the micropattern of biotin and to demonstrate the selective immobilization of proteins by using enhanced green fluorescent protein (EGFP) as a model protein, of which the C-terminus was fused to a core streptavidin (cSA) gene of Streptomyces avidinii. Fluorescence microscopy was used to visualize the pattern of the immobilized protein (EGFP-cSA), and surface plasmon resonance was used to characterize biological activity of the immobilized EGFP-cSA. As a proof-of-principle, we constructed Bacillus spores that displayed EGFP on the spore surface. The results suggest that this strategy is an effective way for fabricating biologically active substrates that are suitable for a wide variety of applications. [Our work was supported by the KOSEF through the Center for Ultramicrochemical Process Systems and by grant No. (R01-2006-000-11175-0) from the Basic Research Program of the Korea Science and Engineering Foundation.] |
| 저자 |
최 솔1, 박태정1, 이경복2, 이상엽1
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| 소속 |
1한국과학기술원 생명화학공학과, 2한국기초과학(연) |
| 키워드 |
streptavidin; Microcontact printing; immobilized protein
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| E-Mail |
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| 원문파일 |
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