| 학회 |
한국고분자학회 |
| 학술대회 |
2007년 가을 (10/11 ~ 10/12, 일산킨텍스) |
| 권호 |
32권 2호 |
| 발표분야 |
생체모방형 고분자재료(의료용 고분자 부문위원회) |
| 제목 |
In vitro chondrogenesis of rabbit mesenchymal stem cells in hydrogel using tissue engineering technique |
| 초록 |
In this study, mesenchymal stem cells (MSCs) from rabbits were encapsulated in a fibrin hydrogel and cultured with or without transforming growth factor-b3 (TGF-b3)-loaded nanoparticles to study the potential for chondrogenesis in a hydrogel scaffold system amenable to minimally invasive implantation. MSCs proliferated well in the hydrogels with TGF-b3 loaded in nanoparticles and TGF-b3 alone compared to TGF-b3-unloaded nanoparticles or fibrin hydrogel alone (scaffold disappeared). Immunohistochemistry revealed that large amounts of type II and decreased collagen type I existed in the TGF-b3 loaded in nanoparticles. Upregulation of aggrecan and type II collagen gene expression compared with the absence of TGF-3 was demonstrated. Type I collagen gene expression decreased from 1 to 4 weeks in the presence of TGF-3. This study demonstrates the ability to encapsulate MSCs with sustained release of growth factors in order to form cartilage-like tissue in vitro in fibrin hydrogel. |
| 저자 |
박근홍 |
| 소속 |
포천 중문 의과대 |
| 키워드 |
mesenchymal stem cells; tissue engineering; hydrogel
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| E-Mail |
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