| 학회 | 한국고분자학회 |
| 학술대회 | 2005년 봄 (04/14 ~ 04/15, 전경련회관) |
| 권호 | 30권 1호, p.658 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Surfce Modification and Fibrovascular Ingrowth of Nonporous and Porous Polyethylene for Anophthalmic Implants |
| 초록 | High density porous polyethylene (PE) has been used successfully for use as anophthalmic socket implants,1,2 facial bone reconstruction, orbital fracture repair, volume augmention, and lower eyelid spacers. It has been suggested that porous implants which undergo fibrovascular tissue ingrowth will have decreased the incidence of migration, infection, and extrusion. Meanwhile, for surface modification of polymers with hydrophilic functional group, acrylic acid (AA) was grafted on the surface of nonporous and porous PE by in situ direct plasma treatment using Ar gas. The surface modification was identified by analyses of ATR-FTIR, SEM, water contact angles, ESCA, and -COOH content by Toluidine Blue O. Recently, some synthetic bioactive ligands such as the peptide, Arg-Gly-Asp (RGD), have been immobilized into polymeric materials to induce specific cell adhesion. In this study, we tried to modify nonporous and porous PE surfaces and then examine in vitro cell adhesion and in vivo fibrovascular ingrowth for anophthalmic implants. The carboxylic acids on PE surfaces were obtained by AA graft plasma polymerization and then readily reacted with RGD. The order of in vitro cell adhesion on the nonporous and porous PE was PE control ＜ PE-g-PAA ＜ PE-g-RGD (Fig. 1). For in vivo rabbit experiment, each implant was inserted between the abdominal muscle layers for four weeks. The in vivo tissue ingrowth was evaluated by cross-sectional area, tissue matiration index stained with hematoxylin and eosin (H&E), and vessel counting with immunohistochemical staining on CD-31. The effect of hydrophilic AA grafting and RGD ligand immobilization on fibrovascular ingrowth into porous PE implants is demonstrated. Fig. 1. WST-1 protein quantity for nonporous and porous PE. References 1. P. De Potter, T. Duprez, and G. Cosnard, Ophthalmology, 107, 1656-1660 (2000). 2. S. M. Blaydon, T. R. Shepler, R. W. Neuhaus, W. L. White, and J. W. Shore, Ophthal. Plast. Reconstr. Surg., 19, 364-371 (2003). |
| 저자 | 양희석1, 박병우2, 안광덕1, 김병수3, 백세현2, 한동근1 |
| 소속 | 1한국과학기술(연), 2고려대, 3한양대 |
| 키워드 | Anophthalmic implants; Porous polyethylene; Surface modification |
