| 학회 | 한국고분자학회 |
| 학술대회 | 2005년 가을 (10/13 ~ 10/14, 제주 ICC) |
| 권호 | 30권 2호 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Chondrocyte Culture on Functional PLLA Scaffolds using Bioreactor System for Tissue Regeneration |
| 초록 | Cartilage implants for potential use in reconstructive or orthopedic surgery can be created by growing isolated cartilage cells (chondrocytes) in vitro on synthetic biodegradable polymer scaffolds. The scaffolds provide specific three-dimensional structures which support cell proliferation and biodegrade in a controlled fashion in parallel to cellular regeneration of cartilaginous tissue. Chondrocyte growth and cartilage matrix regeneration on polymer scaffolds are interdependent and also depend on in vitro tissue culture conditions. Under static culture conditions, cell growth rates are diffusionally limited due to increasing cell mass and decreasing effective implant porosity resulting from cartilage matrix regeneration.1 Optimization of the in vitro culture environment is thus essential for the cultivation of large, clinically useful cartilage implants. It was known that major improvements can be achieved using bioreactors that provide efficient mass transfer and controlled shear rates at the cell and implant surfaces. It suggests that the different condition of shear stress has significant influence on the quality of tissue engineered artificial cartilage. Under cell culture, characterizing the static and dynamic environments and their effects on chondrocyte can help establish direct relationships between pulse power and shear stress.2 We have already surface-modified PLLA nonporous films and porous scaffolds using plasma treatment and acrylic acid (AA) grafting and then examined in vitro cell adhesion in static conditon. In this study, chondrocyte culture on the resulting functional PLLA scaffolds using bioreactor system was investigated and compared to the results in the static condition. References 1. J. C. Hu and K. A. Athanasiou, Biomaterials, 26, 2001-2012 (2005). 2. L. E. Freed, A. P. Hollander, I. Martin, J. R. Barry, R. Langer, and G. Vunjak-Novakovic. Exp. Cell. Res., 240, 58-65 (1998). |
| 저자 | 양희석1, 안광덕1, 김병수2, 한동근1 |
| 소속 | 1한국과학기술(연), 2한양대 |
| 키워드 | Chondrocyte; PLLA Scaffold; Surface Modification; Bioreactor |
