| 초록 |
In this present study, we fabricated microporous PLGA and HA loaded PLGA scaffolds by a emulsion freeze-drying method and seeded chondrocyte cell. Chondrocytes were isolated from the articular cartilage of New Zealand white rabbit and cultured. The chondrocytes were seeded on the HA-PLGA scaffolds and measured at 1, 3, 7, 14 and 21 days by MTT assay. Morphological observation, histology, biological assay for collagen and sGAG, and PCR were performed at each time point 1, 2 and 3 weeks. When we cultured cartilage cell of rabbit in vitro, we observed better to keep the characteristic of cartilage cell in the HA-PLGA scaffolds than that PLGA scaffolds. Scaffolds containing hyaluronic acid (HA) were higher cell viability then only PLGA scaffolds. Study suggests that HA-PLGA scaffold may serve as a potential cell delivery vehicle and a structural basis for in vitro tissue engineered articular cartilage. This work was supported by KMOHW (0405-BO01-0204-0006). |
