| 초록 |
While enormous progress has been made in engineering enzymes, microbial cells, and bioprocessings to produce a variety of renewable fuels and chemicals from sugars derived from recalcitrant lignocellulosic material, the economically viable use of biomass-derived sugars is still a challenge. Thermochemical-based process, so-called pyrolysis can be used to convert biomass into an energy-rich liquid (bio-oil). Pyrolysis of untreated biomass can produce bio-oil that contains up to 12% levoglucosan, which can be concentrated up to 30% after pretreatment. In this work, we aim to engineer Corynebacterium glutamicum, a widely-known industrial bacterium for productions of amino acid and varieties of biochemical, to utilize levoglucosan as a sole carbon source. We selected several leoglucosan kinases and cloned into a CoryneBrick vector for C. glutamicum. Heterologous target genes could be further optimized. This work will broaden the possibility of utilization of biooil as potential feedstocks. |
