| 초록 |
With the advent of LOC(Lab-on-a-chip) and μ-TAS(total analysis system) technology, there has been an increasing demand for efficient methodologies for DNA, protein, and metabolites analyses (biosensing). This presentation describes a strategy of signal registration from micropatterned immunosensors that converts antigen-antibody binding reaction into electrochemical signals. Of the transduction techniques in use such as electrochemical, optical, piezoelectric, etc., amperometric signaling was chosen for the relatively direct application of the microelectronics, which is advantageous for sensor miniaturization and automation. The cyclic voltammetric method, tracking biocatalyzed precipitation of insoluble products onto the sensing surface and subsequent decrement in the electrode area, was used for signal registration. As a model immunosensing with the micro-fabricated device, we have investigated the functionalization of ferritin (antigen) to the PAMAM dendrimer-assisted sensing surface and its biospecific interaction with anti-ferritin antiserum. |
