| 학회 |
한국공업화학회 |
| 학술대회 |
2013년 봄 (05/01 ~ 05/03, ICC JEJU) |
| 권호 |
17권 1호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
Expedite Evolution of Escherichia coli Equipped with Riboselector |
| 초록 |
Although great progress has been made in rational approach, strain improvement still requires the optimization of the biological system itself. Combinatorial approach is, however, limited by the method isolating high producer from the library. In this study, we developed a RNA device to express as a selective phenotype with response to the intracellular biochemical level which is not associated with the growth against the selective pressure. As a model, lysine synthetic pathway was optimized in E. coli. The anaplerotic pathway by ppc was re-optimized by selection of its optimal expression level among the promoter library. By combining RNA device which responds to the intracellular lysine level, optimal ppc expression level for lysine synthesis was easily selected. A physiological study demonstrated that the performance of optimized ppc variant on lysine production compared to its parental strain. |
| 저자 |
양진아1, 서상우1, 정규열2
|
| 소속 |
1Department of Chemical Engineering, 2Department of Chemical Engineering / I-BIO |
| 키워드 |
Strain improvement; evolution; RNA device
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| E-Mail |
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