| 초록 |
Biological systems designs require various dynamic controllers capable of modulating cellular phenotypes to adapt to changing environments. Cellular phenotypes are simultaneously affected by combinations of multiple genes, but it is difficult to intentionally control the expression of these global regulators dynamically. Here, we designed a platform that allows dynamic modulation of the expression of an essential gene. Using this system, comprising on/off genetic switches for several transcriptional regulators that respond to an extracellular stimulus, we successfully demonstrated the dynamic control of the expression of fusA encoding elongation factor G (EF-G). An additional control module in this system that responds to changed external signals was shown to provide the capacity to “switch gears” and reprogram cellular phenotypes with desired timing. |
