| 학회 | 한국화학공학회 |
| 학술대회 | 2007년 봄 (04/19 ~ 04/20, 울산 롯데호텔) |
| 권호 | 13권 1호, p.377 |
| 발표분야 | 생물화공 |
| 제목 | Effect of mechanical strain on in vitro behavior of mesenchymal stem cells |
| 초록 | Mechanical stress is known to be one of the important factors in the regulation of ligament and tendon remodeling The effects of mechanical stress on cells are dependent on the magnitude, duration, and frequency of mechanical stress. Recently the in vitro reconstruction of anterior cruciate ligament (ACL) has been tried using tissue engineering, especially mesenchymal stem cell (MSC) is considered as a good cell source. In this study we tried to find the effect of mechanical stimulus on the differentiation of bone marrow-derived MSC into ACL fibroblast-like cell using a cell training bioreactor imposing cyclic mechanical tension whose parameters were 12 cycles/min, 0.2 Hz, various strain. And MTT assay, FASC analysis, morphology analysis and RT-PCR were performed. it is thought that mechanical cyclic tension induced the loss of stemness of MSC, therefore improved the differentiation into ACL fibroblast-like cell. |
| 저자 | 최경민1, 서영권1, 윤희훈1, 송계용2, 권순용3, 이화성3, 박용순4, 김영진5, 박정극1 |
| 소속 | 1동국대, 2중앙대, 3카톨릭대, 4광주보건대, 5(주)라이프코드 |
| 키워드 | mechanical strain; proliferation; differentiation; mesenchymal stem cells; bone marrow |
| 원문파일 | 초록 보기 |
