| 초록 |
A pattern gel has been designed and developed using sodium hyaluronateand 1,4-butanediol diglycidyl ether by the micro technique. The behavior of osteoblasts in the presence and absence of DMOG and NaB in the gel has been evaluated for its potential application in bone regeneration. FTIR, 13C NMR and TGA and SEM analysis confirmed the formation of pattern on the surface of HA-BDDGE. The property of the gel has been investigated by swelling study. The gel is biocompatible and the bolus drug delivery results indicated that the DMOG containing patterned gel demonstrates a better cell migration ability on the surface than NaB. The pattern gel with NaB or DMOG induced cell clusters formation. In vitro results indicated that the pattern gel showed angiogenic and osteogenic responses with ALP activity and enhanced HIF-1α, and Runx2 levels in the presence of DMOG and NaB in MC3T3 cells. |
