| 학회 |
한국공업화학회 |
| 학술대회 |
2018년 봄 (05/02 ~ 05/04, 대구 엑스코(EXCO)) |
| 권호 |
22권 1호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Improved expression of cell penetrating scFv using an engineered ubiquitin in a cell-free protein synthesis system |
| 초록 |
Antibody is in the core of current research for novel therapeutic and diagnostic agents. In particular, antibodies that can cross cellular membranes have potential applications in controlling disease-related intracellular protein-protein interactions. In this study, we developed an engineered ubiquitin sequence that substantially improves the expression level of cytosol-penetrating antibodies in a cell-free protein synthesis system. The initial codons of a ubiquitin gene was randomized, and the expression level of scFv fused with each of the variant ubiquitin sequences was examined to identify the optimal initial codons. As a result, we were able to select a number of variant ubiquitin clones that significantly improves the expression level of the fused cytosol-penetrating antibodies. |
| 저자 |
장연재1, 박유진1, 이경호1, 이소정1, 임혜진1, 김지선2, 김용성2, 김동명1
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| 소속 |
1충남대, 2아주대 |
| 키워드 |
cytosol-penetrating antibodies; ubiquitin; cell-free protein synthesis system
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| E-Mail |
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