| 학회 |
한국공업화학회 |
| 학술대회 |
2013년 봄 (05/01 ~ 05/03, ICC JEJU) |
| 권호 |
17권 1호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
Engineering thermal property of elastin-like proteins (ELPs) through incorporation of unnatural amino acids in a cell-free protein synthesis system |
| 초록 |
Incorporation of unnatural amino acids into proteins is of particular interest in that it can endow proteins with novel properties that cannot be met with the natural set of 20 amino acids. We adopted and modified a cell-free protein synthesis system to incorporate unnatural amino acids into a variant elastin- like protein. Through the residue-specific substitution of proline with a number of steric analogues, we were able to change the temperature-responding characteristic of the polymer depending on the kinds of substituting amino acids. This study demonstrates the possibility of using cell-free protein synthesis systems for producing engineered bio-materials with expanded amino acid repertoire. |
| 저자 |
오수진1, 이경호1, 원종인2, 윤형돈3, 김동명1
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| 소속 |
1충남대, 2홍익대, 3영남대 |
| 키워드 |
cell-free protein synthesis system; unnatural amino acids; elastin like polypeptides
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| E-Mail |
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