| 초록 |
Butanol and ethanol have high potential as fuel, but acetone has not. In fermentation of Clostridium acetobutylicum, acetone production could make dissipation of carbon source. In order to decrease acetone production, we constructed the predominantly alcohologenic strain from C. acetobutylicum M5, which can hardly produce solvents due to the lack of megaplasmid. We constructed plasmid pIMP1E1AB including aad-ctfAB operon, and transformed into C. acetobutylicum M5. The resulting strain, M5E1AB was observed as predominantly alcohologenic. In batch fermentation studies, co-expression of aad and ctfAB led to formation of 11.1 g/l butanol and 1.7 g/l ethanol with decreased acetone production (0.6 g/L). These results show that the performance of butanol production of M5E1AB was similar to that of ATCC824, but was not in acetone production. |
