| 학회 |
한국재료학회 |
| 학술대회 |
2020년 가을 (11/18 ~ 11/20, 휘닉스 제주 섭지코지) |
| 권호 |
26권 1호 |
| 발표분야 |
B. 나노화학/바이오 재료 분과 |
| 제목 |
Decellularized Liver Extracellular Matrix Loaded Nanocellulose Based Biomaterials for Faster Hemostasis |
| 초록 |
Rapid bleeding control plays predominant role in solving current civilian, military emergency conditions, and prevention of post-operative complications. In this study, a super absorbable freeze dried sponge materials was developed for immediate hemostatic applications by combining extracellular matrix (ECM) hydrogel generated from the intact decellularized porcine liver tissue (E), modified tempo oxidized cellulose nanofiber (T) through EDC/NHS-crosslinking and lawsone (L). The ECM is a good supporting material for synthesis of 3D materials. They still can keep the similar biochemical and functional characteristics of the intact decellularized tissues. The nano cellulose was successfully cross-linked with liver ECM and lawsone through amide and hydrogen bonding. The porosity of developed sponge was reduced after ECM incorporation. Due to soaking-promoting structure, the mechanically stable TLE sponge exhibited unique fluidic absorption potency in in vitro swelling study. In addition, the coagulation tests revealed enhanced platelet adhesion and whole blood absorption by TLE sponge compared to sample devoid of ECM and commercial hemostatic film (Cutanplast®). The enhanced l929 fibroblast cells compatibility allowed the TLE sponge feasible for in vivo experiments which were further confirmed by rabbit femoral artery, rat liver avulsion model and rat tail amputation model. The optimized TLE sponge exhibited rapid hemostatic efficiency by reducing bleeding time and improved blood cells accumulation validated through scanning electron microscopy and gene expression analysis. In conclusion, developed TLE scaffold could be an excellent hemostatic agent for first-aid applications. |
| 저자 |
Tamanna Sultana1, Byong-Taek Lee2
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| 소속 |
1Department of Regenerative Medicine, 2College of Medicine |
| 키워드 |
Nanocellulose; liver ECM; lawsone; hemostasis
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| E-Mail |
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