| 초록 |
In gene therapy, the delivery of gene to the target site is the most important. However, the gene therapy has a disadvantage, off-target effect, of causing inordinate biodistribution to major organs. The off-target effect can cause side effect such as normal cell death and decrease therapeutic effect. To complement for drawback, human mesenchymal stem cells (hMSCs) were used because hMSCs can migrate to tumor site by homing effect. Also, we used polymer as non-viral vector and the polymer was complexed with gene (g/plex). The gene expresses tumor targeted cell death ligands that kills tumor cells through apoptosis. However, the transfection efficiency of the g/plex was lower than viral vector. Therefore, we utilized a photosensitizer to enhanced efficiency of the g/plex transfection into hMSCs. Overall, cell death ligand expressing gene was successfully transfected into hMSCs via complexation and photosensitizer, so that hMSCs were modified to secrete cell death ligands at tumor site. |
