| 초록 |
Cell adhesion to the extracellular matrix (ECM) is directly related to cell survival, migration, cell cycle progression and expression of differentiated phenotype. Therefore, cell behavior can be controlled by manipulating focal adhesion point because cell attachment is controlled by focal adhesion molecules such as fibronectin, laminin and integrin. In order to induce differentiation of stem cell, we prepared nano-scale pillar array modified with focal adhesion protein. Nanoscale features were patterned in the resist using e-beam lithography (EBL) (Elionix, ELS7000) (100eKV). The silicon master was silanized with trichloro(1H, 1H, 2H, 2H-perfluorooctyl)silane in vapor phase then prepolymer of poly (dimethylsiloxane) (PDMS; sylgard 184, Dow-Coring), was poured over an array of holes on the master and cured at 85˚C for 3h and then release from the master. This resulted in arrays of elastic PDMS pillars of 500nm diameter, a height of 1μm, and distances of 3, 5 and 10μm respectively. |
