화학공학소재연구정보센터
학회 한국공업화학회
학술대회 2022년 봄 (05/11 ~ 05/13, 제주국제컨벤션센터(ICC JEJU))
권호 26권 1호
발표분야 포스터-생물공학
제목 Structural and functional investigation of the α and β domain​ fusion in glycerol dehydratase that reduces the oxygen and glycerol inactivation rate
초록 Glycerol dehydratase (GDHt) is a rate-limiting enzyme which converts glycerol into 3-hydroxypropanal (3-HPA) and subsequently transformed into 1,3-propandiol or 3-hydroxypropionic acid. However, GDHt undergoes a mechanism-based inactivation due to the accumulation of an inactive cofactor (B12). The prolonged stability and slower inactivation of the enzyme could be attributed to the terminal residues stabilization of the fused subunits, which distantly influences the Co-N bond between cobalt and dimethylbenzimidazole moiety of B12. Overall, our findings provide novel insights into linker-based enzymatic modulation of GDHt and propose a feasible approach for improving the activity of GDHt in glycerol bioconversion at industrial level.​
저자 나채영, 유태현, Nasir Abdul
소속 아주대
키워드 Enzyme engineering; Structural analysis; Enzyme stability.
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