| 학회 | 한국고분자학회 |
| 학술대회 | 2005년 가을 (10/13 ~ 10/14, 제주 ICC) |
| 권호 | 30권 2호 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Preparation and Release profile of BDNF-loaded PLGA Scaffold |
| 초록 | Recently, brain-derived neurotrophic factor (BDNF) is known neurotrophin family of proteins which have roles in regulating the survival, growth and differentiation of neurones within the developing nervous system. The aim of this study was to investigate release tendency of BDNF in vitro using BDNF's quality. BDNF loaded poly(L-lactide–co-glycolide) (75:25 by mole ratio of lactide to glycolide, PLGA) scaffolds by means of ice-particle leaching method. The release amount of BDNF from BDNF loaded PLGA scaffold were analyzed over a 4 week period in vitro at phosphate buffered saline (PBS), pH 7.4 at 37 ℃. It can be observed the open cell pore structure of porous scaffold and can be easily controlled the pore structure by the controlling of formulation factors resulting in the controlling of the release rate and the release period. The stability of BDNF during the preparation of PLGA scaffold was evaluated by comparing the released amount of total BDNF, assayed BDNF enzyme-linked immunosorbent assay (ELISA). These results suggest that the released BDNF from BDNF loaded PLGA scaffold such as conduit type can be very useful for the nerve regeneration in the neural tissue engineering area. Acknowledgement : This study was supported by KMOICE (N11-A08-1402-05-1-3) |
| 저자 | 김선경1, 이혜란1, 김순희1, 김문석2, 강길선1, 이해방2 |
| 소속 | 1전북대, 2한국화학(연) |
| 키워드 | BDNF; PLGA; scaffold |
