| 학회 |
한국공업화학회 |
| 학술대회 |
2021년 봄 (05/12 ~ 05/14, 부산 벡스코(BEXCO)) |
| 권호 |
25권 1호 |
| 발표분야 |
[생물공학] 팬데믹 이후 및 4차산업 시대의 바이오테크놀로지 |
| 제목 |
Exploration of membrane curvature sensing proteins using spherical supported lipid bilayers |
| 초록 |
Membrane curvature-sensing (MCS) proteins recognize and regulate the morphologies of biological membranes in various cells. In this study, for selective and comprehensive identification of MCS proteins, comparative shotgun proteomic analysis was performed using different sizes of spherical supported lipid bilayer (SSLB), which consists of spherical SiO2 particles covered with a lipid bilayer. Peripheral membrane proteins obtained from normal human dermal fibroblast (NHDF) and human breast cancer (MDA-MB-231) cells were investigated. Statistical quantitative analyses of proteins detected from each SSLB with a different size revealed 23 MCS candidate proteins unique to MDA-MB-231 cells including some previously reported curvature sensors. Further exploration and investigation will lead to the identification of new MCS proteins as well as cancer biomarkers. |
| 저자 |
Masayoshi Tanaka |
| 소속 |
Tokyo Institute of Technology |
| 키워드 |
Proteome analysis; Membrane curvature sensor; Spherical supported lipid bilayer
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| E-Mail |
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