| 학회 |
한국공업화학회 |
| 학술대회 |
2017년 봄 (05/10 ~ 05/12, 광주 김대중컨벤센센터(Kimdaejung Convention Center)) |
| 권호 |
21권 1호 |
| 발표분야 |
생물공학 |
| 제목 |
MALDI-based approaches for comparative analysis of N-linked glycan from Wild-Type and α1,3-Galactosyltransferase Gene Knock-Out Pig Fibroblasts |
| 초록 |
Carbohydrate antigens expressed in porcine cells are known as major barriers to pig-to-human xenotransplantation, but there is no extensive study analyzing N-glycans expressed on the GalT-KO porcine tissues or cells. Thus, we performed MALDI-based relative analysis of totally N-glycans from wild-type (WT) and GalT-KO pig fibroblasts. As a result, we identified and quantified a total of 47 N-glycans. First, the absence of galactose-alpha-1,3-galactose (α-Gal) residues in GalT-KO pig cells was confirmed. Additionally, we identified that the the level of total fucosylated N-glycans from GalT-KO porcine fibroblasts is much higher than from WT porcine fibroblasts and the comparative quantity of the N-glycolylneuraminic acid (NeuGc) antigen is slightly higher in the GalT-KO pigs. Thus, this study could aid a better understanding of the changes of cellular glycan on GalT-KO pig for successful xenotransplantation. |
| 저자 |
황철환1, 박한규1, 송원석2, 안다희1, 정윤이1, 김성민1, 김윤곤1
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| 소속 |
1숭실대, 2서울대 |
| 키워드 |
GalT-KO porcine fibroblast; MALDI; N-glycan; N-glycolylneuraminic acid (NeuGc)
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| E-Mail |
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