| 초록 |
With the development of a new biodegradable and biocompatible polyester composed of 1, 3 propanediol (1, 3-PD), the bioconversion of glycerol and/or glucose to 1, 3-PD has gain much interest. In this biological system, glycerol is dehydrated to 3-HPA by glycerol dehydratase and 3-HPA is then reduced to 1, 3-PD by NADH dependent 1, 3-PD oxidoreductase encoded by dhaT gene. Despite practical importance of DhaT for 1, 3-PD production, there are few studies characterizing DhaT in terms of toxicity of 3-HPA intermediate and reversibility of DhaT. This work aims at kinetic characterization of DhaT from K. pneumoniae. The dhaT gene encoding 1, 3-PD oxidoreductase was cloned from the recently isolated K. pneumoniae J2B, and expressed and purified. Then, the reaction kinetics of DhaT for the forward and backward reactions were investigated using several aldehydes and alcohol as substrates. |
