| 초록 |
Autophagy is a cellular catabolic process that plays significant roles in various diseases such as neurodegenerative diseases, infectious diseases, and cancers. To date, the quest continues to develop an autophagic LC3 detection method with enhanced sensitivity, and which provides a means of quantification to meet the clinical application needs. Here, we present a simple and intuitive autophagy flux measurement by a single gold nanoparticle-based LC3 sensing platform. This nanoplasmonic sensor does not require additional labeling, consisted of only gold nanorods and monoclonal anti-bodies, which enables rapid LC3 quantification within a wide dynamic concentration range, with a detection limit of 64.1 fM. This sensor is the first highly sensitive LC3 quantification nanoplasmonic biosensor that can be used for autophagy measurement. It can serve as an effective tool for determining therapeutic drug dosage in the future. |
