| 학회 |
한국고분자학회 |
| 학술대회 |
2018년 봄 (04/04 ~ 04/06, 대전컨벤션센터) |
| 권호 |
43권 1호 |
| 발표분야 |
대학원생 구두발표 (발표15분) |
| 제목 |
Instantaneous Functionalization of Stellate Gold Nanoparticles for Intracellular Imaging of miRNA |
| 초록 |
We here present a simple one-pot method to prepare stellate gold nanoparticles functionalized with miR-detecting molecular beacons (SGNP-MBs); low pH conditions permitted the rapid-high loading of MBs on the surface of SGNPs. Compared to the conventional gold nanoparticle-based MBs, SGNPs carried a 4.5-fold higher load of MBs and exhibited a 6.4-fold higher cellular uptake. We demonstrated that SGNP-MBs were successfully internalized in human gastric cancer cell lines and could be used to accurately detect and image intracellular miRs in an miR-specific manner. Furthermore, the relative levels of intracellular miRs in three different cell lines expressing miR-10b (high, moderate, and low levels) could be monitored using SGNP-MBs. Consequently, these results indicated that SGNP-MBs could have applications as highly potent, efficient nanoprobes to assess intracellular miR levels in living cells. |
| 저자 |
기지선1, 장은지2, 신무광1, 강병훈1, 한승민1, 이효영1, 허용민2, 함승주1
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| 소속 |
1연세대, 2연세의료원 |
| 키워드 |
branched gold nanoparticles; molecular beacon; miRNA sensing; cancer imaging; nanobiosensor
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| E-Mail |
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