초록 |
We investigated the antibacterial properties of low molecular weight water-soluble chitosan (LMWSC; MW1, MW3, MW5 and MW10) using a combination of approaches, including MIC estimations at 2 different pH values, bactericidal kinetics, calcein-loaded liposomes, depolarization assay, and electron microscopy, as well as an in vivo study utilizing a wound infection and wound healing model. The antibacterial activity of LMWSC was inversely related to pH, with higher activities being observed at lower pH values. Additionally, evaluation of the effect of antibacterial concentrations of LMWSC on the morphology of Bacillus megaterium and Escherichia coli O-157 revealed that it induces filamentation. This study was supported by IPET(Korea Institute of planning & Evaluation for Technology in Food, Agriculture, Forestry & Fisheries) Research Fund in 2009. |