| 학회 | 한국화학공학회 |
| 학술대회 | 2008년 봄 (04/23 ~ 04/25, 제주ICC) |
| 권호 | 14권 1호, p.528 |
| 발표분야 | 생물화공 |
| 제목 | Systems metabolic engineering of Escherichia coli for L-valine and L-threonine production |
| 초록 | By combined genome engineering, transcriptome analysis, and genome-scale metabolic flux analysis, we constructed genetically-defined Escherichia coli strains overproducing L-valine (Val) and L-threonine (Thr). The final engineered E. coli Val overproducing strain showed an impressively high yield of 0.378 g Val per g glucose. The Thr overproducing strain showed even higher yield of 0.393 g Thr per g glucose, and finally produced 82.4 g/l Thr by fed-batch culture. The strategy of systems metabolic engineering reported here can be employed for developing genetically-defined organisms for the efficient production of bioproducts. [This work was supported by the Korean Systems Biology Project of the Ministry of Science and Technology (M10309020000-03B5002-00000). Further supports by the LG Chem Chair Professorship are appreciated]. |
| 저자 | 이상엽1, 박진환1, 이광호2, 김태용1 |
| 소속 | 1KAIST, 2CJ |
| 키워드 | systems metabolic engieering; L-valine; L-threonine |
| 원문파일 | 초록 보기 |
