| 학회 |
한국고분자학회 |
| 학술대회 |
2010년 가을 (10/07 ~ 10/08, 대구 EXCO) |
| 권호 |
35권 2호 |
| 발표분야 |
의료용 고분자 |
| 제목 |
The Effect of PLGA scaffold penetrated DBP solution on the Osteogenic Differentiation of Bone Marrow Stromal Cells |
| 초록 |
Demineralized bone particles(DBP) affects to osteoinductive properties and are very useful clinical trial as natural materials. Poly(lactide-co-glycolide)(PLGA) has been gained FDA approval and used as biomaterials with good biocompatibility. In this study, we evaluated PLGA scaffold penetrated DBP solution on Osteogenesis and proliferation of BMSCs. PLGA scaffold penetrated DBP solution were manufactured that PLGA scaffolds manufactured by solvent casting/salt leaching method soaked in DBP solution. Cellular viability was assayed by 3-(4,5-dimethylthiazole-2-yl)- 2,5-diphenyltertazolium-bromide (MTT) test. Osteoblast differentiation of cells were analyzed by alkaline phosphatase(ALP) activity. RT-PCR was assessed to measure mRNA expression of the genes (ALP, osteocalcine and type I collagen) of osteogenic marker in rBMSCs depending on various content of DBP. This research was supported by WCU(R31-20029), Musculoskeletal Bioorgan Center (0405-BO01-0204-0006) and SCRC (SC 4110). |
| 저자 |
김수진, 김유진, 김형은, 송정은, 이동원, 강길선
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| 소속 |
전북대 |
| 키워드 |
PLGA; DBP; Osteogenic Differentiation
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| E-Mail |
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