| 학회 | 한국화학공학회 |
| 학술대회 | 2011년 가을 (10/26 ~ 10/28, 송도컨벤시아) |
| 권호 | 17권 2호, p.1471 |
| 발표분야 | 생물화공 |
| 제목 | Efficient bioconversion of GABA (γ-aminobutyric acid) using recombinant Escherichia coli strains |
| 초록 | GABA, or gamma-Aminobutyric acid is a non-essential amino acid and a precursor of pyrrolidone, a monomer of nylon 4. GABA can be biosynthesized through the decarboxylation of L-glutamate by glutamate decarboxylase. In this study, the effects of glutamate decarboxylase (gadA, gadB), glutamate/GABA antiporter (gadC) and GABA aminotransferase (gabT) on GABA production were investigated in Escherichia coli. Glutamate decarboxylase was overexpressed alone or with the glutamate/GABA antiporter to enhance GABA synthesis. GABA aminotransferase, which redirects GABA into the TCA cycle, was knock-out mutated. When gadB and gadC were co-overexpressed in the gabT mutant strain, a final GABA concentration of 5.47 g/l was obtained from 10 g/l of monosodium glutamate (MSG), which corresponded to a GABA yield of 89.7%. [This work was supported by the R&D Program of MKE/KEIT (10033199)] |
| 저자 | 이해룡, 홍순호 |
| 소속 | 울산대 |
| 키워드 | Gamma-aminobutyric acid |
| 원문파일 | 초록 보기 |
