| 학회 |
한국화학공학회 |
| 학술대회 |
2011년 봄 (04/27 ~ 04/29, 창원컨벤션센터) |
| 권호 |
17권 1호, p.357 |
| 발표분야 |
생물화공 |
| 제목 |
Peptide biosensor for the molecular recognition of liver biomarker proteins |
| 초록 |
A novel peptide capable of binding to alanine transaminase (ALT) was discovered by biopanning an M13 phage-displayed library. The selection procedure readily converged to a peptide (ALT5-8), with an amino acid sequence WHWRNPDFWYLK. The binding affinity of the peptide-containing phage particles was assessed by ELISA and the specific recognition of ALT was observed with a picomolar binding constant (Kapp= 8.14 pM). To evaluate this system, peptides with the same sequence were chemically synthesized with a C-terminal cysteine and immobilized on a gold surface. A quartz crystal microbalance (QCM) is used as a diagnosis tool during biosensor development, and electrochemical techniques (cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS)) are also used as the detection methods in the biosensor. In this talk, a general method for the facile development of new biosensors for detection of liver biomarker protein targets will be presented. |
| 저자 |
박종필1, Jun Wu2, Kevin Dooley3, Donald M. Cropek2, Alan C. West3, Scott Banta4
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| 소속 |
1대구한의대, 2Department of Chemical Engineering, 3Columbia Univ., 4U.S. Army Engineer Research and Development Center |
| 키워드 |
Biosensor; recognition element; phage display; alanine aminotransferase
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| E-Mail |
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| 원문파일 |
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