| 초록 |
Cartilage tissue engineering is one of the promising approaches via delivering chondrocytes with matrix to the injured site. Two kinds of materials, such as the scaffold and hydrogel, have been used as a matrix but, have their own advantages and limitations. This study attempted to combine the advantages of both for cartilage repair. Poly(L-lactide-co-ε-caprolactone)(PLCL)/gelatin scaffold with good mechanical properties was selected, which was then filled with the heparin hydrogel to mimic the cartilage tissue. PLCL/gelatin scaffold prepared by electrospinning and by a typical salt leaching and heparin hydrogel formed by a Michael-type addition reaction. Then, chondrocytes were injected into the PLCL/G scaffold by using the heparin hydrogel. Mechanical property was analyzed by compression modulus and chondrogenic phenotype was characterized by real-time PCR, DMMB assay, and immunohistochemistry. This work was supported by the Research Center for Biomolecular Nanotechnology, GIST. |
