| 초록 |
Successful immobilization of biological materials on solid substrates by formation of chemical bonding often requires carefully designed self-assembled monolayer of the coupling agents, which acts as a glue layer between substrate and biological materials. In order to address problems associated with immobilization of biomaterials including low surface concentration, non-specific binding and protein denaturation, we investigate the effect of carboxyl/hydroxyl ended coupling agents and BSA for protein immobilization. In this study, a human IgG and GAD were used as probe proteins. We also studied the effects of the surface morphology of coupling layer by using simple linear molecules, dendrimer and mixed monolayers with different mole ratio of the coupling agents. Those are investigated by FT-IR spectroscopy, Surface Plasmon Resonance (SPR) and Atomic Force Microscopy (AFM). |
