| 초록 |
One of the most critical problems of protein engineering is the efficient screening method for the evolutionary approach. In this study, we developed synthetic selection devices using the expression regulators at transcription and translation levels. By combining the product-sensitive promoters and selection markers, a selection device turning the cellular phenotype into selective based on the intracellular concentration of the product. Additionally, intracellular metabolite sensor named “riboselector” as a RNA-based screening device will be presented. The potentials of the platform technology developed in this study for the application to the development of novel enzymes with high catalytic activities |
