| 학회 |
한국공업화학회 |
| 학술대회 |
2017년 봄 (05/10 ~ 05/12, 광주 김대중컨벤센센터(Kimdaejung Convention Center)) |
| 권호 |
21권 1호 |
| 발표분야 |
생물공학 |
| 제목 |
Co-expression of fluorescence protein for assessment of gene expression in a cell-free protein synthesis system |
| 초록 |
The assay of recombinant protein expression generally requires time- and labor-intensive steps. In this study, we demonstrate the use of a cell-free protein synthesis system as a tool for assessment of translational efficiency of different genes. We estimate that the signal intensity from the reporter protein inversely represented the expression level of the co-expressed proteins during the co-expression of a gene of interest with a reporter gene. This allowed us to measure the relative translational efficiencies of different genes in a high-throughput manner. Furthermore, relative translation efficiencies determined by the cell-free co-expression experiments were successfully reproduces when the same genes were deploy into Escherichia coli, suggesting that this method can also be used to predict the yield of protein production a cell-based gene expression. |
| 저자 |
박유진, 이경호, 장연재, 김동명
|
| 소속 |
충남대 |
| 키워드 |
cell free protein synthesis; co-expression; repoter protein
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| E-Mail |
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