| 학회 | 한국화학공학회 |
| 학술대회 | 2008년 봄 (04/23 ~ 04/25, 제주ICC) |
| 권호 | 14권 1호, p.529 |
| 발표분야 | 생물화공 |
| 제목 | Proteomic and physiological analysis of Mannheimia succiniciproducens and its strain improvement |
| 초록 | An efficient succinic acid producer, Mannheimia succiniciproducens MBEL55E, was isolated from bovine rumen of Korean cow. Recently, a genetically engineered succinic acid overproducing mutant, M. succiniciproducens LPK7, was also developed based on full genome sequence. In this study, 2-DE and LC-MS/MS were used to analyze proteome of the mutant cells at the exponential and stationary phases. These results were compared with those of the wild type strain (MBEL55E) to elucidate the global physiological and metabolic changes responsible for succinic acid overproduction. Several features, which correspond to the trend of metabolite production and growth profiles, and indispensable characteristics for further strain improvements will be described in detail. [This work was supported by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MOST) (2005-01294). Further supports by the LG Chem Chair Professorship, IBM SUR program, Microsoft, and by the KOSEF through the Center for Ultramicrochemical Process Systems are appreciated.] |
| 저자 | 이정욱1, 송효학1, 최 솔1, 유종신2, 이상엽1 |
| 소속 | 1KAIST, 2KBSI |
| 키워드 | Mannheimia succiniciproducens; proteome; succinic acid |
| 원문파일 | 초록 보기 |
