| 초록 |
3-hydroxypropionic acid is one of the valuable platform chemicals and widely used in industrial application. As chemical synthesis of 3-HP requires expensive starting materials and high energy consuming process, its production through microorganism based bio-process has been developed. Although one of the recent reports showed commercially available 3-HP production in Escherichia coli, with heterologous enzyme expression and process optimization, there is still room for improvement on its productivity and titer. In addition, coenzyme B12 is the key material for the bio-process production of 3-HP. In this research, glycerol which is a waste from bio-fuel production process was used as a substrate as well as sole carbon source and novel pathway was introduced and reconstructed in Escherichia coli for 3-HP production without any coenzyme B12 supplement. Heterologous genes encoding pathway enzymes and the enzyme reactivase for 3-HP production were expressed under constitutive strong promoters and maximized 5’-untranslated regions. This work was supported by the Advanced Biomass R&D Center. |
