| 학회 |
한국화학공학회 |
| 학술대회 |
2013년 가을 (10/23 ~ 10/25, 대구 EXCO) |
| 권호 |
19권 2호, p.2377 |
| 발표분야 |
Biointerfaces for biotechnology and bioengineering symposium |
| 제목 |
Bio-inspired Silification for Biointerface Design |
| 초록 |
Silicatein (SIL) involved in biosilification in diatoms has high identity and similarity with cathepsin L. We obtained novel hypothetical cathepsin-like protein (CAT) from Nematostella vectensis. This CAT expressed in E. coli displayed not only protease activity but also silica condensing activity. To increase the silica forming activity, some residues including cysteine in active site were changed into silicatein conserved residues. The mutant silicatein-like cathepsin (SLC) showed increased expression level in E. coli and silica forming activity comparable to that of SIL and decreased protease activity as compared to CAT. SIL, CAT and SLC fused with GST were arrayed on GSH-coated plate and catalyzed the formation of silica layer in the presence of TEOS as substrate at ambient and neutral conditions. During biosilification, GFP or HRP was immobilized on silica surface by simple adding it. Immobilized proteins retained their activity and were released gradually. This immobilization technique can be applied to form biocompatible silica coating for diagnostic, catalytic and matrix for tissue cultures, and surface treatment for biosensor. |
| 저자 |
백승필, 기미란, 여기백, 장의경, 민기하, 유영하
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| 소속 |
고려대 |
| 키워드 |
Biosilification; Silicatein; Cathepsin; Silicatein-like Cathepsin
|
| E-Mail |
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| 원문파일 |
초록 보기 |
