| 학회 | 한국화학공학회 |
| 학술대회 | 2007년 가을 (10/26 ~ 10/27, 한국과학기술원) |
| 권호 | 13권 2호, p.1717 |
| 발표분야 | 생물화공 |
| 제목 | Magnetic Nanoparticles Applied in Simultaneous Detection of Multiple DNA Targets |
| 초록 | Multiplexed detection of PCR-based biotinylated oligonucleotide targets has been performed with streptavidin (STA) magnetic nanoparticle and various fluorescence dye-labeled probe DNA. In this study, sexually transmitted diseases (STDs) genes, especially Chlamydia trachomatis, Neisseria gonorrhoeae and Ureaplasma urealyticum were employed as a model system. STA magnetic nanoparticles were characterized by UV-Vis microscopy and transmission electron microscopy (TEM), and the multiple-step process was analyzed by fluorescence microscopy. Furthermore, magnetic nanoparticle could be employed to separate the reacted samples from unreacted ones in whole process, and control the DNA hybridization process. References: 1. Taihua Li, Hyun Gyu Park, Hee-Seung Lee, and Seong-Ho Choi, Nanotechnology 15(10) s660-s663 (2004) 2. Huachang Lu, Guangshun Yi, Shuying Zhao, Depu Chen, Liang-Hong Guo and Jing Cheng, J. Mater. Chem. 14 1336 (2004) 3. Yun Wei, Charles Cao, Rongchao Jin, and Chad A. Mirkin, Science 297 1536 (2002) |
| 저자 | 이태화, Rongzhan Fu, 박현규 |
| 소속 | 한국과학기술원 |
| 키워드 | Multiple detection; DNA; magnetic nanoparticle |
| 원문파일 | 초록 보기 |
